Introduction: Rubella, also known as German Measles or Three-Day Measles, is a mild, self-limiting, vaccine-preventable infection that closely resembles measles in presentation, including fever and rash. Aim: To confirm the clinically suspected cases of Rubella virus. Methodology:      The present study was a laboratory-based descriptive observational study conducted at the WHO National Rubella Laboratory, Department of Microbiology, SMS Medical College, Jaipur, Rajasthan, from January 2023 to December 2023. Result: Our study highlights a significant association between rubella seropositivity and age (p=0.000), with all positive cases occurring in infants, while no significant association was found with gender or measles status. CONCLUSION: Strengthening vaccination coverage and surveillance is crucial to controlling and eliminating Measles and Rubella, especially in vulnerable infant populations